Table S2. Loss of tbc1 is not lethal; adults homozygous for the null allele are viable and morphologically normal, although the failure of approximately one fourth of embryos from homozygous mothers to develop suggests some reduction in female fertility, a phenotype that could be related to the border cell migration defects previously reported [ 15 ]. The salivary gland is specified by the coordinate action of three homeobox-containing transcription factors: Sex combs reduced ScrExtradenticle Exd and Homothorax Hth [ 12 ]. Green arrows indicate lacZ expression from the balancer chromosome; Black arrow indicates the salivary gland. Table S1.
FlyBase: a database for drosophila genetics and molecular biology. Dmel\fkh. Species. D. melanogaster. Name.
fork head. Annotation Symbol. CG Fkh promotes terminal as opposed to segmental development. In the absence of fkh, this developmental switch does not occur.
The nuclear localization of the. fkh. Organism. Drosophila melanogaster (Fruit fly). Status. Unreviewed- Annotation score: Experimental evidence at transcript leveli.
Video: Fkh drosophila fly Fruit fly and its life-cycle under the microscope
Development of Tbc1 antiserum and testing of transgenic lines. We used a second chromosome fkh -Gal4 line that drives expression in the SG, but to variable levels in different cells.
Asterisks indicate species included in the alignment. View Article Google Scholar 9. Loss of Tbc1d23 in stimulated macrophages increased both the levels and duration of cytokine production, whereas overexpression of Tbc1d23 did the opposite.
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|This group further demonstrated decreases in the average velocity of retrograde and anterograde transport of NYP-GFP labeled vesicles and of lysosomes using lysotracker when Tbc1d23 was knocked down in fibroblasts.
In contrast to the endogenous protein see figure 4overexpressed FKH is localized in the nucleus also under conditions of high TOR and insulin signaling. J Biol Chem — The correct 1.
B Effect of FKH overexpression on cell size.
fkh fork head [ (fruit fly)]. Gene ID: FKH in the gut in mediating anti-aging effects of reduced insulin/insulin growth factor. Fkh maintains. We find that ectopic expression and targeted knockdown of FKH in Animals; Drosophila Proteins/metabolism*; Drosophila melanogaster.
EF Closeup of A, B. The effects on cell size were similar to those on organismal size and are displayed in Figure 2the quantitative and statistical analysis of cell size phenotypes is shown in Figure 3.
A similar correlation was observed when using the expression of FKH target gene candidates as a readout. Nat Genet. Our studies reveal that both loss and overexpression of tbc1 results in SG secretion defects and irregularities in the lumenal membrane.
A line with the insertion on chromosome 3 was then crossed to a line expressing flippase and the restriction enzyme I-Sce1 for excision and linearization of the transgenic construct, as described in [ 26 ].
Maruyama R, Andrew DJ.
FELLAINI IMAGE AT MAN UTD
|EMBO J — View Article Google Scholar 6. A Effect of FKH knockdown on cell size. Global analysis of patterns of gene expression during Drosophila embryogenesis.
A and C On a protein-rich yeast paste diet, co-expression of TSC1 and TSC2 in cell clones in the larval fatbody leads to a strong reduction in cell size. Nat Rev Genet — In this instance, GFP antibody was used to detect Tbc1.